TEL&FAX 092-642-6582(研究室) 〒812-8582 福岡市東区馬出3-1-1 薬学研究院1号館２階 |
2024
原著論文
1. Noguchi, K., Imahori, D., Nishiura, R., Nuntawong, P., Putalun, W., Tanaka, H., Morimoto, S., Sakamoto, S. “Advanced quality assessment of Sanshishi (Gardenia jasminoides Ellis) and Kampo medicines using a monoclonal antibody against geniposide” Fitoterapia, in press, 2024.
2. Ochi, A.*, Nuntawong, P.*, Chaingam, J., Ota, T., Juengwatanatrakul, T., Putalun, W., Shoyama, Y., Tanaka, H., Morimoto, S., Sakamoto, S. *These authors contributed equally to this work. “Simultaneous rapid detection of glycyrrhizin and sennoside A in Daiokanzoto samples by lateral flow immunoassay” Phytochem. Anal., in press, 2024.
3. Ochi, A.*, Fujii, S.*, Ohta, T., Uto, T., Nuntawong, P., Tanaka, H., Morimoto, S., Sakamoto, S. *These authors contributed equally to this work. “Highly sensitive indirect competitive enzyme-linked immunosorbent assay based on a monoclonal antibody against saikosaponin b2 for quality control of Kampo medicines containing Bupleuri radix” J. Nat. Med., 78(1), 160-168, 2024.
2023年
総説等
1. Taura, F., Sirikantaramas, S., Sakamoto, S., Tanaka, H., Morimoto, S., Shoyama, Y. “Comprehensive review of cannabis including cannabinoid brain function” Med. Res. Arch., in press, 2023.
原著論文
1. Noguchi, K., Imahori, D., Kido, Y., Tanaka, H., Morimoto, S., Sakamoto, S. “Quality assessment method for Chinpi, dried Citrus spp. peel and its-derived Kampo medicines using specific monoclonal antibody against hesperidin” Phytochem. Anal., 34(6), 652-660, 2023.
2. Yusakul, G., Thammakhet-Buranachai, C., Poorahong, S., Sakamoto, S., Makkliang, F. “An eco-friendly method using deep eutectic solvents immobilized in a microcrystal cellulose-polyvinyl alcohol sponge for parabens analysis in food samples” Microchemical J., 191, 108758, 2023.
3. Juengsanguanpornsuk, W., Poopanee, N., Krittanai, S., Sakamoto, S., Tanaka, H., Putalun, W., Yusakul, G. “Immunoaffinity separation of miroestrol and deoxymiroestrol from Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham using fragment antigen-binding antibody produced via E. coli” Phytochem. Anal., 34(6), 632-640, 2023.
4. Krittanai, S., Choonong, R., Butdapheng, K., Jabsanthia, J., Sakamoto, S., Putalun, W. “Construction of a monoclonal antibody against glabridin (2G4) and development of an enzyme-linked immunosorbent assay” Phytochem. Anal., 34(5), 571-579, 2023.
5. Sae-Foo, W., Yusakul, G., Kitisripanya, T., Nuntawong, P., Sakamoto, S., Putalun, W. “Comparative stability and analytical performance of anti-miroestrol recombinant antibody in different cassettes” Appl. Microbiol. Biotechnol., 107, 2887-2896, 2023.
6. Sakdamas, A., Makkliang, F., Putalun, W., Juengwatanatrakul, T., Kanchanapoom, T., Sakamoto, S., Yusakul, G. “Analysis of canthin-6-one alkaloids derived from Eurycoma spp. by micellar liquid chromatography and conventional high-performance liquid chromatography: a comparative evaluation” RSC Adv., 13(9), 6317-6326, 2023.
7. Chuphol, N., Nuttikarn Nokkaew, N., Makkliang, F., Sae-Foo, W., Phaisan, S., Putalun, W., Sakamoto, S., Yusakul, G. “Immunochromatographic assay for miroestrol and deoxymiroestrol, its cross-reactivity, and application in Pueraria mirifica (white Kwao Krua) analysis” Phytochem. Anal., 34(4), 421-430, 2023.
学会発表
(国際)1 Chaingam, J., Huy, L.V., Yusakukl, G., Noguchi, K., Morimoto, S., Sakamoto, S.
“Monoclonal antibody production against majonoside-R2, the major bioactive compound of Vietnamese ginseng”
71st International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA), Dublin, Ireland, July 2-5, 2023, P317
2 Noguchi, K., Ochi, A., Nuntawong, P., Fujii, S., Tanaka, H., Morimoto, S., Sakamoto, S.
“Production of specific monoclonal antibody against marker compound, hesperidin, geniposide, and saikosaponin b2 for the quality control of crude drugs”
71st International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA), Dublin, Ireland, July 2-5, 2023, P319
[Gold Oral Presentation Award]
3 Noguchi, K., Imahori, D., Kido, Y., Nuntawong, P., Tanaka, H., Morimoto, S., Sakamoto, S.
“Immunoassay for the quality control of Citrus unshiu peel”
PST Conference 2023, Phitsanulok, Thailand, June 15-16, 2023, OPAPC061
4 Ochi, A., Tanaka, H., Morimoto, S., Sakamoto, S.
“Establishment of a quality evaluation method for Daio-kanzo-to using fluorescent-labeled antibody”
PST Conference 2023, Phitsanulok, Thailand, June 15-16, 2023, OPAPG062
5 Sakamoto, S., Minami, K., Nuntawong, P., Yusakul, G., Putalun, W., Tanaka, H., Fujii, S., Morimoto, S.
“Detection of kwakhurin via bioimprinted protein”
PST Conference 2023, Phitsanulok, Thailand, June 15-16, 2023, PPAPG066
[優秀発表賞（口頭発表）]
1. 尾崎 孝汰、田山 順恵、Poomraphie Nuntawong、安部 寛子、森元 聡、坂元 政一
"世界アンチドーピング機構が策定する禁止表国際基準におけるヒゲナミン収載の妥当性”
日本生薬学会 第69回年会、東北医科薬科大学 小松島キャンパス、2023年9月9−10日、O08-4
[優秀発表賞（ポスター発表）]
2. 後藤 理沙、森元 聡、坂元 政一
"組換えストリクトシジン合成酵素を用いたストリクトシジンの合成及び安定性に関する研究”
日本生薬学会 第69回年会、東北医科薬科大学 小松島キャンパス、2023年9月9−10日、2P-64
3. 藤井 俊輔、越智 昭裕、太田 智絵、宇都 拓洋、田中 宏幸、森元 聡、坂元 政一
"サイコサポニンb2に対するモノクローナル抗体の作製および間接競合ELISAへの応用”
日本生薬学会 第69回年会、東北医科薬科大学 小松島キャンパス、2023年9月9−10日、2P-09
4. 越智 昭裕、田中 宏幸、正山 征洋、森元 聡、坂元 政一
"大黄甘草湯の迅速な品質評価法の確立を目指した二成分同時分析系の構築”
日本薬学会 第143年会、札幌、2023年3月25−28日、26E4-pm01S
5. Poomraphie Nuntawong, 田山 順恵, 森元 聡, 坂元 政一
“Development of aptamer-based detection method to quantify berberine in Kampo medicine samples”
日本薬学会 第143会、札幌、2023年3月25−28日、国際交流シンポジウム、次世代薬学アジアシンポジウム3、IS02-S3-05
2022年
総説等
1. Wenger, T., Watanabe, K., Sasaki, Y., Kanazawa, K., Shimizu, K., Sirikantaramas, S., Shoyama, Y., Taura, F., Morimoto, S., Shoyama, Y. “Overview of Cannabis including Kampo medicine and therapy for treatment of dementia: a review” Front. Pharmacol.,12, 713228, 2022.
2. Nuntawong, P., Putalun, W., Tanaka, H., Morimoto, S. Sakamoto, S. “Lateral flow immunoassay for small-molecules detection in phytoproducts: a review” J. Nat. Med., 76(3), 521-545, 2022.
原著論文
1. Sakamoto, S., Minami, K., Nuntawong, P., Yusakul, G., Putalun, W., Tanaka, H., Fujii, S., Morimoto, S. “Bioimprinting as a receptor for detection of kwakhurin” Biomolecules, 12(8), 1064, 2022.
2. Juengsanguanpornsuk, W., Kitisripanya, T., Boonsnongcheep, P., Yusakul, G., Srisongkram, T., Sakamoto, S., Putalun, W. “Improvement in the binding specificity of anti-isomiroestrol antibodies by expression as fragments under oxidizing conditions inside the SHuffle T7 E. coli cytoplasm” Biosci. Biotechnol. Biochem., in press, 2022.
3. Kongpol, K., Sermkaew, N., Makkliange, F., Khongphan, S., Chuaboon, L., Sakdamas, A., Sakamoto, S., Putalun, W., Yusakul, G. “Extraction of curcuminoids and ar-turmerone from turmeric (Curcuma longa L.) using hydrophobic deep eutectic solvents (HDESs) and application as HDES-based microemulsions” Food Chem., 396, 133728, 2022.
4. Nuntawong, P., Horikawa, T., Tanaka, H., Morimoto, S., Sakamoto, S. “Activated carbon-based immunochromatographic strip test for the rapid qualitative analysis of swertiamarin and sweroside” J. AOAC Int., 105(5), 1460-1467, 2022.
5. Krittanai, S., Pichetpongtorn, P., Sakamoto, S., Putalun, W. “Monoclonal antibody-based immunoassay for specific quantification of licochalcone A: an active chalcone in licorice” Food Agric. Immunonol., 33(1), 220-234, 2022.
6. Ochi, A., Yoritate, M., Miyamoto, T., Usui, K., Yusakul, G., Putalun, W., Tanaka, H., Hirai, G., Morimoto, S., Sakamoto, S. “Harringtonine ester derivatives with enhanced antiproliferative activities against HL-60 and Hela cells” J. Nat. Prod., 85(2), 345-351, 2022.
学会発表
(国際)1. Ochi, A., Miyamoto, T., Yoritate, M., Tanaka, H., Hirai, G., Sakamoto, S., Morimoto, S.
“Synthesis of harringtonine ester derivatives possessing potent anti-leukemia activities”
4th International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2022) (Online Meeting), February 7-8, 2022 (poster)
2. Noguchi, K., Fujii, S., Tanaka, H., Sakamoto, S., Morimoto, S.
“Production of anti-hesperidin monoclonal antibody and establishment of quantitative analysis method by ELISA”
4th International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2022) (Online Meeting), February 7-8, 2022 (poster)
3. Nuntawong, P., Tanaka, H., Sakamoto, S., Morimoto, S.
“Prussian blue nanoparticles based lateral flow assay for detection of the sport-prohibited-compound, higenamine”
4th International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2022) (Online Meeting), February 7-8, 2022 (poster)
4. Sakamoto, S., Minami, K., Yusakul, G., Putalun, W., Tanaka, H., Morimoto, S.
“Quantitative analysis of glycyrrhizin using rapid magnetic particles-based enzyme immunoassay for the quality control of Glycyrrhiza spp.”
4th International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2022) (Online Meeting), February 7-8, 2022 (poster)
1. 野口 幹太、新川 裕生、Poomraphie Nuntawong、坂元 政一、森元 聡
"ヘスペリジンに対するモノクローナル抗体の作製及びLateral Flow Immunoassay (LFA)への応用”
日本生薬学会 第68回年会、松山大学文京キャンパス、(オンライン)、2022年9月10−11日、O05-2
2. Jiranan Chaingam、坂元 政一、Thaweesak Juengwatanatrakul、Gorawit Yusakul、Tripetch Kanchanapoom、Waraporn Putalun
"Chaparrinon: Eurycoma harmandiana Pierre identical marker and the studies of anti-inflammatory effec”
日本生薬学会 第68回年会、松山大学文京キャンパス、(オンライン)、2022年9月10−11日、1P-03
3. Poomraphie Nuntawong、坂元 政一、森元 聡
"Selection, characterization, and application of the aptamer for berberine detection method”
日本生薬学会 第68回年会、松山大学文京キャンパス、(オンライン)、2022年9月10−11日、1P-40
4. 淵上 里緒、越智 昭裕、田中 宏幸、宇都 拓洋、正山 征洋、坂元 政一、森元聡
"甘草の育種研究に向けた抗グリチルリチン小型化抗体(GC-scFv)遺伝子の特徴付け”
日本生薬学会 第68回年会、松山大学文京キャンパス、(オンライン)、2022年9月10−11日、2P-06
5. 坂元 政一
"ドーピング禁止物質、健康食品成分及び生薬成分の特異的検出の開発”
日本法中毒学会 第41年会、香川、2022年6月25−26日、ランチョンセミナー
6. 野口 幹太、藤井 俊輔、田中 宏幸、坂元 政一、森元 聡
"抗ヘスペリジンモノクローナル抗体の作製及び間接競合ELISAの確立”
日本薬学会 第142年会、名古屋、（オンライン）、2022年3月25−28日、28J-am01S
7. Poomraphie Nuntawong, 堀川 泰暉, 田中 宏幸, 坂元 政一, 森元 聡
“Determination of swertiamarin and related compounds in the gastrointestinal diseases folk medicine, Swertia japonica Makino using immunoassays”
日本薬学会 第142年会、名古屋、（オンライン）、2022年3月25−28日、28J-am02S
8. 内山 裕貴、坂元 政一、Gorawit Yusakul、Benyakan Pongkitwitoon、Waraporn Putalun、田中 宏幸、森元 聡
"大豆イソフラボン配糖体の検出を目指したオープンサンドイッチ蛍光イムノアッセイの確立”
日本薬学会 第142年会、名古屋、（オンライン）、2022年3月25−28日、27PO1-am1-13
2021年
総説等
1. 坂元 政一 "生薬成分に対する抗体と免疫学的測定法” Foods & Food Ingredients J. Japan, 226(1), 263-274, 2021.
2. 九州大学薬学府附属薬用植物園、『医歯薬学系博物館事典』、落合知子編、雄山閣, 176 頁, 2021年
原著論文
1. Makkliang, F., Juengsanguanpornsuk, W., Phaisan, S., Sakdamas, A., Putalun, W., Sakamoto, S., Yusakul, G. “Transformation of Pueraria candollei var. mirifica phytoestrogens using immobilized and free β-glucosidase, a technique for enhancing estrogenic activity” RSC Adv., 11, 32607-32076, 2021.
2. Makkliang, F.*, Siriwarin, B.*, Yusakul, G., Phaisan, S., Sakdamas, A., Chuphol, N., Putalun, W., Sakamoto, S. *These authors contributed equally to this work. “Biocompatible natural deep eutectic solvent-based extraction and cellulolytic enzymes mediated transformation of Pueraria mirifica isoflavones, a sustainable approach for increasing health-bioactive constituents” Bioresour. Bioprocess., 8(1), 76, 2021.
3. Sakamoto, S., Uchiyama, H., Yusakul, G., Kyokong, N., Pongkitwitoon, B., Putalun, W., Tanaka, H., Morimoto, S. “Open sandwich fluorescence-linked immunosorbent assay for detection of soy isoflavone glycosides.” Food Chem., 361, 129829, 2021.
4. Sae-Foo, W., Krittanai, S., Juengsanguanpornsuk, W., Yusakul, G., Kitisripanya, T., Sakamoto, S., Putalun, W. “Enzyme-linked immunosorbent assay using fragment antigen-binding (Fab) antibody for determination of deoxymiroestrol, a potent phytoestrogen from Pueraria candollei.” Food Agric. Immunol., 32(1), 336-348, 2021.
5. Sae-Foo, W., Krittanai, S., Juengsanguanpornsuk, W., Yusakul, G., Sakamoto, S., Putalun, W. “Fragment antigen-binding (Fab) antibody-based lateral flow immunoassay for rapid and sensitive detection of potent phytoestrogen, deoxymiroestrol.” J. Nat. Med., 75(4), 1043-1049, 2021.
6. Krittanai, S., Buraphaka, H., Sakamoto, S., Putalun, W. “Enhancement of nutraceutical property in licorice callus cultures using sample pretreatment strategy.” Int. J. Food Sci. Technol., 56, 5843-5852, 2021.
7. Phaisan S., Makkliang F., Putalun W., Sakamoto S., Yusakul G. “Development of a colorless Centella asiatica extract using a natural deep eutectic solvent (NADES) and microwave-assisted extraction (MAE) optimized by response surface methodology.” RSC Adv., 11, 8741-8750, 2021.
8. Rattanapisit, K., Kitisripanya, T., Konyanee, A., Sae-Foo, W., Burapapiruin, A., Putalun, W., Sakamoto, S., Phoolcharoen, W., Yusakul, G. “Plant-made antibody against miroestrol: A new platform for expression of full-length immunoglobulin G against small-molecule targets in immunoassays.” Plant Cell Rep., 40(4), 723-733, 2021.
9. Minami, K., Yusakul, G., Fujii, S., Putalun, W., Tanaka, H., Sakamoto, S., Morimoto, S. “Rapid magnetic particles-based enzyme immunoassay for the quality control of Glycyrrhiza spp. based on glycyrrhizin content.” Fitoterapia, 148, 104794, 2021.
10. Nuntawong, P., Ochi, A., Chaingam, J., Tanaka, H., Sakamoto, S., Morimoto, S. “The colloidal gold nanoparticle based-lateral flow immunoassay for fast and simple detection of plant-derived doping agent, higenamine.” Drug Test Anal., 13, 762-769, 2021.
11. Juengsanguanpornsuk, W., Yusakul, G., Kitisripanya, T., Krittanaia, S., Juengwatanatrakul, T., Sakamoto, S., Putalun, W. “Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with a high-performance liquid chromatography.” J. Pharm. Biomed. Anal., 192, 113674, 2021
12. Nuntawong, P., Horikawa, T., Ochi, A., Wada, S., Tsuneura, Y., Tanaka, H., Sakamoto, S., Morimoto, S. “A monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay to quantify swertiamarin and its related compounds in Swertia japonica Makino.” Phytochem. Anal., 32, 512-520, 2021.
13. Nuntawong, P., Lohseethong, K., Juengwatanatrakul, T., Yusakul, G., Putalun W., Tanaka, H., Sakamoto, S., Morimoto, S. “Competitive immunochromatographic test strips for the rapid semi-quantitative analysis of the biologically active bitter glycoside, amarogentin.” J. Immunoassay Immunochem., 42(1), 48-61, 2021.
14. Phaisan, S., Yusakul, G., Nuntawong, P., Sakamoto, S., Putalun, W., Morimoto, S., Tanaka, H. “Immunochromatographic assay for the detection of kwakhurin and its application for identification of Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham.” Phytochem. Anal., 32, 503-511, 2021.
15. Sakamoto, S.*, Morita, Y.*, Yusakul, G., Putalun, W., Tanaka, H., Morimoto, S. *These authors contributed equally to this work. “Molecular cloning and characterization of type III polyketide synthase from Plumbago zeylanica” J. Asian Nat. Prod. Res., 23(5), 478-490, 2021.
学会発表
(国際)1. Nuntawong, P., Tanaka, H., Sakamoto, S., Morimoto S.
“Development of icELISA for the detection of the prohibited doping agent higenamine”
The 11th JSP/CSP/KSP Joint Symposium on Pharmacognosy (Virtual conference), September 18-19, 2021 (poster), P3-57
1. 井上 花奈、坂元 政一、森元 聡
"植物のアサの成分 THCA に対する解毒機構に関する研究”
日本生薬学会 第67回年会、星薬科大学 (東京)（オンライン）、2021年9月19−20日、P2-02
2. 越智 昭裕、寄立 麻琴、臼井 一晃、宮本 智文、平井 剛、田中 宏幸、坂元 政一、森元 聡
"ハリントン誘導体のHL-60とHela細胞に対する増殖阻害活性に関する研究”
日本生薬学会 第67回年会、星薬科大学 (東京)（オンライン）、2021年9月19−20日、O01-3
3. 坂元 政一、衛藤 亮太、Poomraphie Nuntawong、Gorawit Yusakul、Waraporn Putalun、藤井 俊輔、田中 宏幸、森元 聡
"磁気ビーズイムノアッセイを用いたプエラリア・ミリフィカに含有するクワクリンの定量分析法の確立”
日本薬学会 第141年会、広島国際会議場、JMSアステールプラザ(広島)（オンライン）、2021年3月26−29日、27P02-044
2020年
総説等
1. Uto, T., Fujii, S., Sakamoto, S., Ohta, T., Shoyama, Y. “Applications of monoclonal antibodies against natural compounds for functional analysis of crude drugs.” Curr. Pharmacol. Rep., 6, 192-201, 2020.
原著論文
1. Nuntawong, P., Tanaka, H., Sakamoto, S., Morimoto, S. “ELISA for the detection of the prohibited doping agent higenamine in medicinal plants.” Planta Med., 86(11), 760-766, 2020.
2. Sakamoto, S., Eto, R., Nuntawong, P., Yusakul, G., Juengwatanatrakul, T., Putalun, W., Fujii, S. Tanaka, H., Morimoto, S. “Kwakhurin-magnetic particles conjugates enabled fast enzyme immunoassay for the detection of kwakhurin in Pueraria candollei.” Phytochem. Anal., 31(6), 930-936, 2020.
3. Krittanai, S., Putalun, W., Sakamoto, S., Tanaka, H., Juengwatanatrakul, T., Yusakul, G. “Expression of actively soluble antigen-binding fragment (Fab) antibody and GFP fused Fab in the cytoplasm of the engineered Escherichia coli.” Mol. Biol. Rep., 47(6), 4519-4529, 2020.
4. Phaisan, S., Yusakul, G., Nuntawong, P., Sakamoto, S., Putalun, W., Morimoto, S., Tanaka, H. “Honey as a solvent for the green extraction, analysis, and bio-conversion of daidzin from Pueraria candollei var. mirifica root.” Pharmacogn. Mag., 16(71), 524-530, 2020.
5. Phaisan, S., Yusakul, G., Sakdamas, A., Taluengjit, N., Sakamoto, S., Putalun, W. “A green and effective method using oils to remove chlorophyll from Chromolaena odorata (L.) R.M. King & H. Rob.” Songklanakarin J. Sci. Technol., 42(5), 1084-1090, 2020.
6. Kitisripanya, T., Sermpradit, W., Sakamoto, S., Tanaka, H., Putalun, W. “An estimated quantitative lateral flow immunoassay for determination of artesunate using monoclonal antibody.” Biomed. Chromatogr., 34(2), e4718, 2020.
学会発表
(国際)1. Ochi, A., Miyamoto, T., Yoritate, M., Tanaka, H., Sakamoto, S., Morimoto, S.
“Synthesis and evaluation of anti-leukemia activity of harringtonine ester derivatives”
3rd International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2020) (Online Meeting), November 2-4, 2020 (poster) E-poster 5
2. Minami, K., Yusakul, G., Fujii, S., Sakamoto, S., Morimoto, S.
“Bioimprinting technique for the quantitative analysis of kwakhurin”
3rd International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2020) (Online Meeting), November 2-4, 2020 (poster) E-poster 4
3. Sakamoto, S., Eto, R., Nuntawong, P., Yusakul, G., Juengwatanatrakul, T., Putalun, W., Fujii, S., Tanaka, H., Morrimoto, S.
“Development of magnetic particles-based enzyme immunoassay for the detection of kwakhurin in Pueraria candollei”
3rd International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2020) (Online Meeting), November 2-4, 2020 (poster) E-poster 2
2019年
総説等
1. 坂元 政一 "大麻の生理活性物質を効率よくつくる” Nature, 567(2019)123-126 解説, 現代化学 5月号, 578, 13-14, 2019.
原著論文
1. Li, R.S., Fukumori, R., Takeda, T., Song, Y., Morimoto, S., Kikura-Hanajiri, R., Yamaguchi, T., Watanabe, K., Aritake, K., Tanaka, Y., Yamada, H., Yamamoto, T., Ishii, Y. “Elevation of endocannabinoids in the brain by synthetic cannabinoid JWH-018: mechanism and effect on learning and memory.” Sci. Rep., 9(1), 9621, 2019.
2. Yusakul, G., Sakamoto, S., Tanaka, H., Morimoto, S. “Modification of first constant domain of heavy chain (CH1) enabled effective folding of functional anti-forskolin antigen-binding fragment (Fab) for sensitive quantitative analysis.” Biotechnol. Prog., 35(4), e2822, 2019.
3. Chaingam, J., Kitisripanya, T., Krittanai, S., Sakamoto, S., Tanaka, H., Putalun, W. “Development of a simple and rapid method for detection of isomiroestrol in Pueraria candollei by an immunochromatographic strip test.” J. Nat. Med., 73(3), 577-583, 2019.
4. Yusakul, G., Sakamoto, S., Chanpokapaiboon, K., Tanaka, H., Morimoto, S. “Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid.” Phytochem. Anal., 30(6), 653-660, 2019.
5. Pongkitwitoon, B., Boonsnongcheep, P., Kitisripanya, T., Yusakul, G., Sakamoto, S., Tanaka, H., Morimoto, S., Putalun, W. “Preparation of a highly specific single chain variable fragment antibody targeting miroestrol and its application in quality control of Pueraria candollei by enzyme-linked immunosorbent assay.” Phytochem. Anal.,30(6), 600-608, 2019.
6. Tan, H., Zhao, C., Zhu, Q., Katakura, Y., Tanaka, H., Ohnuki, K., Shimizu, K. “Ursolic acid, isolated from the leaves of loquat (Eriobotrya japonica) inhibited osteoclast differentiation through targeting exportin 5.” J. Agric. Food Chem.,67(12), 3333-3340, 2019.
7. Myose, T., Shirakawa, A., Irie, K., Yamashita, Y., Nakano, T., Takase, Y., Matsuo, K., Satho, T., Tuchihashi, R., Kinjo, J., Tanaka, H., Morimoto, S., Funada, M., Sano, K., Mishima, K. “Δ9-Tetrahydrocannabinol elicited 22-kHz ultrasonic vocalization changes after air puff stimulus through CB1 receptor in adult rats.” Neurosci. Lett., 701, 132-135, 2019.
8. Igawa, T., Kishikawa, S., Abe, Y., Yamashita, T., Nagai, S., Shiroishi, M., Shinozaki, C., Tanaka, H., Tozaki-Saitoh, H., Tsuda, M., Inoue, K., Ueda, T. “Evidence for detection of rat P2X4 receptor expressed on cells by generating monoclonal antibodies recognizing the native structure.” Purinergic Signal., 15(1), 27-35, 2019.
9. Shindo, N., Fuchida, H., Sato, M., Watari, K., Shibata, T., Kuwata, K., Miura, C., Okamoto, K., Hatsuyama, Y., Tokunaga, K., Sakamoto, S., Morimoto, S., Abe, Y., Shiroishi, M., Caaveiro, J.M.M., Ueda, T., Tamura, T., Matsunaga, N., Nakao, T., Koyanagi, S., Ohdo, S., Yamaguchi, Y. Hamachi, I., Ono, M., Ojida, A. “Selective and reversible modification of kinase cysteines with chlorofluoroacetamides.” Nat. Chem. Biol., 15(3), 250-258, 2019.
10. Yusakul, G.*, Togita, R.*, Minami, K.*, Chanpokapaiboon, K., Juengwatanatrakul, T., Putalun, W., Tanaka, H., Sakamoto, S., Morimoto, S. *These authors contributed equally to this work. “An indirect competitive enzyme-linked immunosorbent assay toward standardization of Pueraria candollei based on its unique isoflavonoid, kwakhurin.” Fitoterapia, 133, 23-28, 2019.
11. Komaikul, J., Kitisripanya, T., Likhitwitayawuid, K., Sritularak, B., Tanaka, H., Putalun, W. “Improvement of stilbenoid production by 2-hydroxypropyl-β-cyclodextrin in white mulberry (Morus alba L.) callus cultures.” Nat. Prod. Res., 33(19), 2762-2769, 2019.
12. Sakamoto, S., Wada, S., Morita, Y., Yamaguchi, T., Tanaka, H., Morimoto, S. “Magnetic particles-based enzyme immunoassay for rapid determination of secoiridoid glycoside, amarogentin.” Talanta, 194, 731-736, 2019.
13. Inyai, C., Boonsnongcheep, P., Komaikul, J., Sritularak, B., Tanaka, H., Putalun, W. “Alginate immobilization of Morus alba L. cell suspension cultures improved the accumulation and secretion of stilbenoids.” Bioprocess Biosyst. Eng., 42(1), 131-141, 2019.
学会発表
(国内)1. 南 圭、磨田 瑠衣、Gorawit Yusakul、田中 宏幸、坂元 政一、森元 聡
"プエラリア・ミリフィカ特有成分“Kwakhurin”の高感度イムノアッセイの確立”
日本生薬学会 第66回年会、北里大学 (東京)、2019年9月21−23日、1C-01
2. 内山 裕貴、坂元 政一、田中 宏幸、森元 聡
"ダイズイソフラボン配糖体の検出を目的としたオープンサンドイッチ蛍光イムノアッセイの開発”
日本生薬学会 第66回年会、北里大学 (東京)、2019年9月21−23日、1C-02
3. 坂元 政一、Gorawit Yusakul、田中 宏幸、森元 聡
"重鎖定常領域置換による抗フォルスコリンFab の巻き戻し効率の向上と定量分析への応用”
日本生薬学会 第66回年会、北里大学 (東京)、2019年9月21−23日、P-27
4. 森保 歩惟、小松 頌平、坂元 政一、田中 宏幸、森元 聡
"三分割GFP を用いたハリントニンの新規免疫学的蛍光測定法の開発”
日本生薬学会 第66回年会、北里大学 (東京)、2019年9月21−23日、P-42
2018年
総説等
1. Sakamoto, S., Putalun, W., Vimolmangkang, S., Phoolcharoen, W., Shoyama, Y., Tanaka, H., Morimoto, S. “Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites.” J. Nat. Med., 72(1), 32-42, 2018.
原著論文
1. Chanpokapaiboon, K., Khoonrit, P., Yusakul, G., Juengwatanatrakul, T., Putalun, W., Tanaka, H., Sakamoto, S., Morimoto S. “A recombinant Fab antibody against kwakhurin as a tool for sensitive indirect competitive ELISA.” Curr. Pharm. Biotechnol., 19(14), 1170-1176, 2018.
2. Yusakul, G., Sakamoto, S., Tanaka, H., Morimoto, S. “Improvement of heavy and light chain assembly by modification of heavy chain constant region 1 (CH1): Application for construction of anti-paclitaxel fragment antigen-binding (Fab).” J. Biotechnol., 288, 41-47, 2018.
3. Krittanai, S., Kitisripanya, T., Udomsin, O., Tanaka, H., Sakamoto, S., Juengwatanatrakul, T. Putalun, W. “Development of a colloidal gold nanoparticle-based immunochromatographic strip for the one-step detection of miroestrol and puerarin.” Biomed. Chromatogr., 32(11), e4330, 2018.
4. Sakamoto, S., Wada, S., Tanaka, H., Morimoto, S. “Sensitive quantitative analysis of the bitter glycoside, amarogentin by specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay.” RSC Adv., 8(31), 17410-17416, 2018.
5. Udomsin, O., Krittanai, S., Kitisripanya, T., Tanaka, H., Putalun, W. “A new highly selective and specific anti-puerarin polyclonal antibody for determination of puerarin using a Mannich reaction hapten conjugate.” Pharmacogn. Mag., 13(Suppl 4), S845-S851, 2018.
6. Yusakul, G., Kitisripanya, T., Juengwatanatrakul, T., Sakamoto, S., Tanaka, H., Putalun, W. “Enzyme linked immunosorbent assays for total potent estrogenic miroestrol and deoxymiroestrol of Pueraria candollei, a Thai herb for menopause remedy.” J. Nat. Med., 72(3), 641-650, 2018.
7. Pongkitwitoon, B.*, Sakamoto, S. *, Nagamitsu, R., Putalun W., Tanaka, H., Morimoto, S. * These authors contributed equally to this work. “A monoclonal antibody-based enzyme-linked immunosorbent assay for determination of homoharringtonine.” Planta Med., 84(14), 1038-1044, 2018.
8. Limsuwanchote, S., Putalun, W., Tanaka, H., Morimoto, S., Keawpradub, N., Wungsintaweekul, J. “Development of an immunochromatographic strip incorporating anti-mitragynine monoclonal antibody conjugated to colloidal gold for kratom alkaloids detection.” Drug Test. Anal., 10, 1168-1175, 2018.
9. Sakamoto, S., Miyamoto, T., Usui, K., Tanaka, H., Morimoto, S. “Sodium-periodate mediated harringtonine derivatives and their antiproliferative activity against HL-60 acute leukemia cells.” J. Nat. Prod., 81(1), 34-40, 2018.
10. Cui, Q., Tanaka, H., Shoyama, Y., Ye, H.T., Li, F., Tian, E.W., Wu, Y.S., Chao, Z. “Development of a competitive time-resolved fluoroimmunoassay for paclitaxel.” Phytochem. Anal., 29(3), 284-289, 2018.
11. Yusakul, G., Sakamoto, S., Nuntawong, P., Tanaka, H., Morimoto S. “Different expression systems resulted in varied binding properties of anti-paclitaxel single chain variable fragment antibody clone 1C2.” J. Nat. Med., 72(1), 310-316, 2018.
学会発表
(国際)1. Pongkitwitoon, B., Sakamoto, S., Nagamitsu, R., Waraporn, P., Tanaka, H., Morimoto S.
“Development of immunoassay for analysis of homoharringtonine in plant samples”
22nd International Phytopharm Congress 2018, Wadenswil, Switzerland, June 25-27, 2018 (poster) No.73
2. Sakamoto, S., Miyamoto, T., Usui, K., Tanaka, H., Morimoto S.
“Antiproliferative activity of sodium periodate-mediated harringtonine derivatives against HL-60 acute leukemia cells”
The 5th International Conference on Current Drug Development 2018 (CDD 2018) and The 3rd International Conference on Herbal and Traditional Medicine 2018 (HTM 2018), PSU Conference Hall, Songkhla, Thailand, May 23-25, 2018 (poster) PH-01
3. Chaingam, J., Kitisripanya T., Sakamoto, S., Tanaka, H., Morimoto, S., Putalun, W.
“Construction and expression of single-chain fragment against miroestrol in silkworm”
The 5th International Conference on Current Drug Development 2018 (CDD 2018) and The 3rd International Conference on Herbal and Traditional Medicine 2018 (HTM 2018), PSU Conference Hall, Songkhla, Thailand, May 23-25, 2018 (poster) PH-38
4. Krittanai S., Yusakul G., Sakamoto, S., Tanaka, H., Putalun, W.
“Expression and characterization of anti-miroestrol fragment antigen binding in E. coli”
The 5th International Conference on Current Drug Development 2018 (CDD 2018) and The 3rd International Conference on Herbal and Traditional Medicine 2018 (HTM 2018), PSU Conference Hall, Songkhla, Thailand, May 23-25, 2018 (poster) PH-39
5. Juengsanguanpornsuk, W., Kitisripanya, T., Yusakul, G., Srisongkram, T., Krittanai S., Sakamoto, S., Tanaka, H., Putalun, W.
“Expression and characterization of antigen binding fragment (Fab) against isomiroestrol in E.coli ShuffleR”
The 5th International Conference on Current Drug Development 2018 (CDD 2018) and The 3rd International Conference on Herbal and Traditional Medicine 2018 (HTM 2018), PSU Conference Hall, Songkhla, Thailand, May 23-25, 2018 (poster) PH-40
1. 森田 優衣、坂元 政一、田中 宏幸、森元 聡
“セイロンマツリ由来ポリケタイド合成酵素(polyketide synthase: PKS)に関する研究”
第35回 日本薬学会九州支部大会、九州大学(福岡)、2018年11月17−18日、1-B-11
2. 坂元 政一
“植物二次代謝産物の免疫化学的分析法の開発”
日本生薬学会 第65回年会、安田女子大学(広島)、2018年9月16−17日、2A-AL4
学術奨励賞受賞講演
3. 松浦 圭祐、坂元 政一、田中 宏幸、森元 聡
“コカ由来III型ポリケタイド合成酵素についての研究”
日本生薬学会 第65回年会、安田女子大学(広島)、2018年9月16−17日、2P-76
4. 杉山 菜摘、坂元 政一、田中 宏幸、森元 聡
“コガネバナ由来ペルオキシダーゼに対する中和抗体の作製及びその応用”
日本生薬学会 第65回年会、安田女子大学(広島)、2018年9月16−17日、2B-08
5. 森田 優衣、坂元 政一、田中 宏幸、森元 聡
“セイロンマツリ由来ポリケタイド合成酵素(polyketide synthase: PKS)に関する研究”
日本生薬学会 第65回年会、安田女子大学(広島)、2018年9月16−17日、1C-03
6. 衛藤 亮太、和田 伸司、山口 朋子、坂元 政一、田中 宏幸、森元 聡
“磁気ビーズを用いた苦味配糖体「アマロゲンチン」の免疫化学的分析法の開発”
日本生薬学会 第65回年会、安田女子大学(広島)、2018年9月16−17日、1A-05
年会優秀発表賞受賞
7. 赤木 麻里、明瀬 孝之、入江 圭一、近藤 布由美、伊賀 歩美、山下 郁太、松尾 宏一、林 稔展、佐藤 朝光、土橋 良太、金城 順英、森元 聡、田中 宏幸、佐野 和憲、三島 健一
“白金系抗がん剤oxaliplatin誘発性末梢神経障害ラットに対する大麻成分Δ9-tetrahydrocannabinolによる治療効果”
日本薬学会 第138年会、石川県立音楽堂他(金沢)、2018年3月25−28日、26PA-am197
8. 坂元 政一、宮本 智文、臼井 一晃、田中 宏幸、森元 聡
“過ヨウ素酸ナトリウムによるハリントニン誘導体の作出及びヒト前骨髄性白血病細胞HL60に対する増殖阻害活性”
日本薬学会 第138年会、石川県立音楽堂他(金沢)、2018年3月25−28日、27PA-pm213
2017年
原著論文
1. Okubo, S, Uto, T., Goto, A., Tanaka, H., Nishioku, T., Yamada, K., Shoyama, Y. “Berberine induces apoptotic cell death via activation of caspase-3 and-8 in HL-60 human leukemia cells: nuclear localization and structure-activity relationships.” Am. J. Chin. Med., 45(7), 1497-1511, 2017.
2. Supasuteekul, C., Tadtong, S., Putalun, W., Tanaka, H., Likhitwitayawuid, K., Tengamnuay, P., Sritularak, B. “Neuritogenic and neuroprotective constituents from Aquilaria crassna leaves.” J. Food Biochem., 41(3), e12365, 2017.
3. Egashira, N., Koushi, E., Myose, T., Tanoue, A., Mishima, K., Tsuchihashi, R., Kinjo, J., Tanaka, H., Morimoto, S., Iwasaki, K. “Role of vasopressin V1a receptor in 9-tetrahydrocannabinol-induced cataleptic immobilization in mice.” Psychopharmacology (Berl), 234,(23-24), 3475-3483, 2017.
4. Yusakul, G.*, Nuntawong P.*, Sakamoto, S., Ratnatilaka Na Bhuket P, Kohno, T., Kikkawa, N., Rojsitthisak P. Shimizu, K., Tanaka, H., Morimoto, S. * These authors contributed equally to this work. “Bacterial expressions of single-chain variable fragment (scFv) antibody against ganoderic acid A, a cost-effective approach for its quantitative analysis using the scFv based enzyme-linked immunosorbent assay.” Biol. Pharm. Bull., 40(10), 1767-1774, 2017.
5. Paudel, M.K., Sakamoto S., Tanaka, H. Morimoto, S. “An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide.” J. Nat. Med., 71(4), 703-710, 2017.
6. Kitisripanya, T., Inyai, C., Komaikul, J., Krittanai, S., Juengwatanatrakul, T., Sakamoto, S., Tanaka, H., Morimoto, S., Putalun, W. “A lateral flow, colloidal gold-based immunoassay for rapid detection of miroestrol in samples of White Kwao Krua, a phytoestrogen-rich plant.” J. Nat. Med., 71(4), 659-664, 2017.
7. Paudel, M.K., Shirota, O., Sakamoto S., Tanaka, H., Morimoto, S. “An immunochromatographic assay for rapid detection of salvinorin A.” J. Immunoassay Immunochem., 38(4), 438-448, 2017.
8. Paudel, M.K., Sakamoto S., Huy, L.V., Tanaka, H., Miyamoto, T., Morimoto, S. “The effect of varying the peptide linker length in a single chain variable fragment antibody against wogonin glucuronide.” J. Biotechnol., 251, 47-52, 2017.
9. Kitisripanya, T., Inyai, C., Krittanai, S., Likhitwitayawuid, K., Sritularak, B., Ploypradith, P., Tanaka, H., Morimoto, S., Putalun W. “A monoclonal antibody-based immunoassay for the determination of oxyresveratrol from Artocarpus lacucha Buch.-Ham.” J. Nat. Med., 71(3), 523-530, 2017.
10. Sakamoto, S., Yusakul, G., Tsuneura, Y., Putalun, W., Usui, K., Miyamoto, T., Tanaka, H., Morimoto, S. “Sodium periodate-mediated conjugation of harringtonine enabling the production of a highly specific monoclonal antibody, and the development of a sensitive quantitative analysis method.” Analyst, 142, 1140-1148, 2017.
11. Sakamoto, S., Nagamitsu R., Yusakul, G., Miyamoto, T., Tanaka, H., Morimoto, S. “Ultrasensitive immunoassay for monocrotaline using monoclonal antibody produced by N, N’-carbonyldiimidazole mediated hapten-carrier protein conjugates.” Talanta, 168, 67-72, 2017.
12. Kitisripanya, T., Krittanai, S., Udomsin, O. Juthasit, K., Komaikul, J., Juengwatanatrakul, T., Sakamoto, S., Tanaka, H., Morimoto, S., Putalun, W. “Development of an enzyme-linked immunosorbent assay for determination of miroestrol using an anti-miroestrol monoclonal antibody.” Planta Med., 83(10), 855-861, 2017.
13. Morita, I., Oyama, H., Yasuo, M., Matsuda, K., Katagi, K., Ito, A., Tatsuda, H., Tanaka, H., Morimoto, S., Kobayashi, N. “Antibody fragments for on-site testing of cannabinoids generated via in vitro affinity maturation.” Biol. Pharm. Bull., 40, 174-181, 2017.
14. Kitisripanya, T., Jutathis, K., Inyai, C., Komaikul, J., Udomsin, O., Tanaka, H., Putalun, W. “Development of an enzyme-linked immunosorbent assay for the detection of isomiroestrol, an identical marker, in White Kwao Krua using a monoclonal antibody.” J. Pharm. Biomed. Anal., 137, 229-234, 2017.
15. Sakamoto, S., Yusakul, G., Nuntawong, P., Kitisripanya, T., Putalun, W., Miyamoto, T., Tanaka, H., Morimoto, S. “Development of an indirect competitive immunochromatographic strip test for rapid detection and determination of anticancer drug, harringtonine.” J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 1048, 150-154, 2017.
16. Kohno, T.*, Hai-Bang, T.*, Zhu, Q., Amen, Y.M., Sakamoto, S., Tanaka, H., Morimoto, S., Shimizu, K. *These authors contributed equally to this work. “Tubulin polymerization stimulating-activity of Ganoderma triterpenoids.” J. Nat. Med., 71(2), 457-462, 2017.
17. Paudel, M.K., Sakamoto S., Huy, L.V., Tanaka, H., Miyamoto, T., Takano A., Morimoto, S. “Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody.・h J. Immunoassay Immunochem., 38(5), 457-470, 2017.
18. Sookying, S., Pekthong, D., Oo-Puthinan, S., Phrompittayarat, W., Putalun, W., Tanaka, H., Xing, J., Zhan, Z., Khorana, N., Nuengchamnong, N., Ingkaninan, K. “Immunochromatographic determination of bacopaside I in biological samples.” J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 1040, 60-66, 2017.
学会発表
(国際)1. Sakamoto, S.
“Immunoassays for cephalotaxus alkaloids, harringtonine and antiproliferative activity of its derivatives”
5th International Conference on Pharmaceuticals, Nutraceuticals and Cosmetic Science (IPNaCS 2017), Bangkok, Thailand, August 3-4, 2017 (invited talk) IL-11
2. Sakamoto, S., Nuntawong, P., Yusakul, G., Ratnatilaka Na Bhuket, P., Kohno, T., Kikkawa, N., Rojsitthisak, P., Shimizu, K., Tanaka, H., Morimoto S.
“The expression of anti-ganoderic acid A single chain variable fragment antibody using Escherichia coli expression systems and their reactivity”
THE JSPS-NRCT FOLLOW-UP SEMINAR 2017 AND 33RD INTERNATIONAL ANNUAL MEETING IN PHARMACEUTICAL SCIENCES (JSPS-NRCT 2017 AND IAMPS 33), Bangkok, Thailand, March 2-3, 2017 (poster) PP-2
3. Sakamoto, S.
“Sodium periodate-mediated conjugation of harringtonine enabling the production of a highly specific monoclonal antibody, and the development of a sensitive quantitative analysis method”
THE JSPS-NRCT FOLLOW-UP SEMINAR 2017 AND 33RD INTERNATIONAL ANNUAL MEETING IN PHARMACEUTICAL SCIENCES (JSPS-NRCT 2017 AND IAMPS 33), Bangkok, Thailand, March 2-3, 2017 (invited talk) IL-2
1. 和田 伸司、坂元 政一、田中 宏幸、森元 聡
“苦味配糖体アマロゲンチンに対する迅速分析法の開発”
日本生薬学会 第64回年会、東邦大学 習志野キャンパス(千葉)、2017年9月9−10日、1C-13
2. 小松 頌平、坂元 政一、Gorawit Yusakul、Putalun Waraporn、田中 宏幸、森元 聡
“Cephalotaxus属由来ハリントニ・唐ﾌ一本鎖抗体の作製及びその応用研究”
日本生薬学会 第64回年会、東邦大学 習志野キャンパス(千葉)、2017年9月9−10日、1C-14
2016年
原著論文
1. Taura, F., Iijima, M., Yamanaka, E., Takahashi, H., Kenmoku H., Saeki, H., Morimoto, S., Asakawa, Y., Kurosaki, F., Morita, H. “A novel class of plant type III polyketide synthase involved in orsellinic acid biosynthesis from Rhododendron dauricum.” Front. Plant Sci., 7, 1452, 2016.
2. Sakamoto, S., Kikkawa, N., Kohno, T., Shimizu, K., Tanaka, H., Morimoto, S. “Immunochromatographic strip assay for detection of bioactive Ganoderma triterpenoid, ganoderic acid A in Ganoderma lingzhi.” Fitoterapia, 114, 51-55, 2016.
3. Jutathis, K., Kitisripanya, T., Udomsin, O., Inyai, C., Sritularak, B., Tanaka, H., Putalun, W. “An enzyme-linked immunosorbent assay for genistein 7-O-[α-rhamnopyranosyl-(1→6)]-β-glucopyranoside determination in Derris scandens using a polyclonal antibody.” Phytochem. Anal., 27(6), 336-342, 2016.
4. Chao, Z., Cui, Q., Tian, E., Zeng, W., Cai, X., Li, X., Tanaka, H., Shoyama, Y., Wu, Y. “Ultrasensitive time-resolved fluoroimmunoassay for saikosaponin a in Chaihu (Bupleuri Radix).” PLoS One, 11(3), e0151032., 2016.
5. Sakamoto, S.*, Kohno, T.*, Shimizu, K., Tanaka, H., Morimoto, S. *These authors contributed equally to this work.
“Detection of ganoderic acid A in Ganoderma lingzhi by an indirect competitive enzyme-linked immunosorbent assay” Planta Med., 82(8), 747-751, 2016.
Selected as Highlight Articles of this issue
6. Yusakul, G., Sakamoto, S., Tanaka, H., Morimoto, S. “Efficient expression of single chain variable fragment antibody against paclitaxel using Bombyx mori nucleopolyhedrovirus bacmid DNA system and its characterizations.” J. Nat. Med.,70(3),592-601, 2016.
7. Yusakul, G., Sakamoto, S., Pongkitwitoon, B., Tanaka, H., Morimoto, S. “Effect of linker length between variable domains of single chain variable fragment antibody against daidzin on its reactivity.” Biosci. Biotechnol. Biochem., 80(7), 1306-1312, 2016.
8. Sakamoto, S.*, Nagamitsu, R.*, Matsuura, Y., Tsuneura, Y., Kurose, H., Tanaka, H., Morimoto, S. *These authors contributed equally to this work. “A new approach of indirect enzyme-linked immunosorbent assay for determination of D-glutamic acid through in situ conjugation” J. Immunoassay Immunochem., 37(3), 296-306, 2016.
9. Kitisripanya, T., Jutathis, K., Inyai, C., Komaikul, J., Udomsin, O., Yusakul, G., Tanaka, H., Putalun, W. “Anti-miroestrol polyclonal antibodies: a comparison of immunogen preparations used to obtain desired antibody properties.” J. Nat. Med., 70(2), 296-299, 2016.
10. Yusakul, G., Sakamoto, S., Juengwatanatrakul, T., Putalun, W., Tanaka, H., Morimoto, S. “Preparation and application of a monoclonal antibody against the isoflavone glycoside daidzin using a Mannich reaction-derived hapten conjugate.” Phytochem. Anal., 27(1), 81-88, 2016.
11. Sakamoto, S., Yusakul, G., Pongkitwitoon, B., Tanaka, H., Morimoto, S. “Colloidal gold-based indirect competitive immunochromatographic assay for rapid detection of bioactive isoflavone glycosides daidzin and genistin in soy products.” Food Chem., 194, 191-195, 2016.
学会発表
(国際)1. Kitisripanya, T., Komaikul, J., Jutathis, K., Udomsin, O., Tanaka, H., Putalun W.
“An enzyme-linked immunoassay using anti-miroestrol monoclonal antibody for quality control of Pueraria candollei products”
The 9th Joint Natural Products Conference 2016 in Copenhagen, Tivoli Hotel & Congress Center, Copenhagen, Denmark, July 24-27, 2016 (poster) P1082
2. Jutathis, K., Kitisripanya, T., Udomsin, O., Inyai, C., Sritularak, B., Tanaka, H., Putalun, W.
“An enzyme-linked immunosorbant assay for genistein 7-O-[α-rhamnopyranosyl-(1→6)]-β-glucopyranoside determination in Derris scandens by using a polyclonal antibody”
The 9th Joint Natural Products Conference 2016 in Copenhagen, Tivoli Hotel & Congress Center, Copenhagen, Denmark, July 24-27, 2016 (poster) P1085
3. Komatsu, S., Sakamoto, S., Yusakul, G., Putalun, W., Miyamoto, T., Tanaka, H., Morimoto, S.
“Development of highly sensitive immunological techniques for determination of cephalotaxus alkaloids, harringtonine”
The 9th Joint Natural Products Conference 2016 in Copenhagen, Tivoli Hotel & Congress Center, Copenhagen, Denmark, July 24-27, 2016 (poster) P777
4. Sakamoto, S., Yusakul, G., Nagamitsu, R., Tsuneura, Y., Putalun, W., Miyamoto, T., Tanaka, H., Morimoto, S.
“Development of highly sensitive immunological techniques for determination of cephalotaxus alkaloids, harringtonine”
The 9th Joint Natural Products Conference 2016 in Copenhagen, Tivoli Hotel & Congress Center, Copenhagen, Denmark, July 24-27, 2016 (poster) P125
5. Yusakul, G., Sakamoto, S., Juengwatanatrakul, T., Putalun, W., Tanaka, H., Morimoto, S.
“Production and characterization of monoclonal antibody against the isoflavone glycoside daidzin”
The 4th Current Drug Development International Conference (CDD 2016)”, Phuket Graceland Resort & Spa, Patong Beach, Phuket, Thailand, June 1-3, 2016 (oral) P39−40
Awarded Best Oral Presentation Award
6. Tanaka, H.
“Development and applications of immunoassays for natural products”
The 6th International Conference on Natural Products for Health and Beauty (NATPRO 6), Khon Kaen, Thailand, January 21-23, 2016 (invited) I8
7.Jie, B., Tanaka, C., Tanaka, H., Dohgu, S., Watanabe T., Miyamoto, T.
“Search for new medicinal seeds to prevent the diabetic microvascular diseases”
The 6th International Conference on Natural Products for Health and Beauty (NATPRO 6), Khon Kaen, Thailand, January 21-23, 2016 (oral) O27
8. Yamamoto, C., Sakamoto, S., Tanaka, H., Morimoto, S.
“Cloning, expression, and purification of polyketide synthase of Erythrooxylum coca”
The 6th International Conference on Natural Products for Health and Beauty (NATPRO 6), Khon Kaen, Thailand, January 21-23, 2016 (poster) P13
9. Sakamoto, S., Yusakul, G., Nagamitsu, R., Tsuneura, Y., Miyamoto, T., Tanaka, H., Morimoto, S.
“Production of highly specific monoclonal antibody against representative cephalotaxine alkaloids, harringtonine and its application to sensitive quantitative/qualitative analyses”
The 6th International Conference on Natural Products for Health and Beauty (NATPRO 6), Khon Kaen, Thailand, January 21-23, 2016 (poster) P97
Awarded Excellence Poster Presentation Award
10. Nagamitsu, R., Sakamoto, S., Tanaka, H., Morimoto, S.
“Production of monoclonal antibodies against pyrrolizidine alkaloid, retronecine and its potential applications”
The 6th International Conference on Natural Products for Health and Beauty (NATPRO 6), Khon Kaen, Thailand, January 21-23, 2016 (poster) P98
Awarded Very Good Poster Presentation Award
1. 橘川 奈生、坂元 政一、河野 俊享、清水 邦義、田中 宏幸、森元 聡
“抗ganoderic acid Aモノクローナル抗体を用いた免疫クロマトグラフィーの確立”
日本生薬学会 第63回年会、富山国際会議場(富山)、2016年9月24−25日、2C-06
2. 坂元 政一、Yusakul Gorawit、長光 梨花、常浦 裕未、Putalun Waraporn、宮本 智文、田中 宏幸、森元 聡
“セファロタキサス属アルカロイド、ハリントニンの高感度免疫定量法の確立”
日本生薬学会 第63回・N会、富山国際会議場(富山)、2016年9月24−25日、2P-28
2015年
総説等
1. Sakamoto, S., Tanaka, H., Morimoto, S. “Towards the prophylactic and therapeutic use of human neutralizing monoclonal antibodies for Middle East respiratory syndrome coronavirus (MERS-CoV)” Ann. Trans. Med., 3(3), 35, 2015.
原著論文
1. Komaikul, J., Kitisripanya, T., Tanaka, H., Sritularak, B., Putalun, W. “Enhanced mulberroside A production from cell suspension and root cultures of Morus alba using elicitation.” Nat. Prod. Commun., 10(7), 1253-1256, 2015.
2. Inyai, C., Komaikul, J., Kitisripanya, T., Tanaka, H., Sritularak, B., Putalun, W. “Development of a rapid immunochromatographic strip test for the detection of mulberroside A.” Phytochem. Anal., 26(6), 423-427, 2015.
3. Yusakul, G., Udomsin, O., Tanaka, H., Morimoto, S., Juengwatanatrakul, T., Putalun, W. “Enzyme-linked immunosorbent assay by enhanced chemiluminescence detection for the standardization of estrogenic miroestrol in Pueraria candollei Graham ex Benth.” Luminescence, 30(5), 568-575, 2015.
4. Sakamoto, S.*, Yusakul, G.*, Pongkitwitoon, B., Paudel, M.K., Tanaka, H., Morimoto, S. *These authors contributed equally to this work. “Simultaneous determination of soy isoflavone glycosides, daidzin and genistin by monoclonal antibody-based highly sensitive indirect competitive enzyme-linked immunosorbent assay” Food. Chem., 169, 127-133, 2015.
学会発表
(国際)1. Yusakul, G., Sakamoto, S., Tanaka, H., Morimoto, S.
“Construction and characterization of the antigen-binding antibody fragment against paclitaxel”
The 1st International Conference on Herbal and Traditional Medicine (HTM 2015), Khon Kaen, Thailand, January 28-30, 2015, P_21, Isan Journal of Pharmaceutical Sciences, 10, 282, 2015.
2. Kohno, T., Sakamoto, S., Tanaka, H., Morimoto, S.
“Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against ganoderic acid A”
The 1st International Conference on Herbal and Traditional Medicine (HTM 2015), Khon Kaen, Thailand, January 28-30, 2015, P_52, Isan Journal of Pharmaceutical Sciences, 10, 297, 2015.
Awarded Best Poster Presentation
3. Sakamoto, S., Putalun, W., Pongkitwitoon, B., Shoyama, Y., Tanaka, H., Morimoto, S.
“Modulation of plumbagin production in Plumbago zeylanica using a single-chain variable fragment antibody against plumbagin”
The 1st International Conference on Herbal and Traditional Medicine (HTM 2015), Khon Kaen, Thailand, January 28-30, 2015, Invited Speaker, Isan Journal of Pharmaceutical Sciences, 10, 24, 2015.
4. Tanaka, H.
“Development of immunoassay using monoclonal antibody and recombinant antibody for natural products”
The 1st International Conference on Herbal and Traditional Medicine (HTM 2015), Khon Kaen, Thailand, January 28-30, 2015, Invited Speaker, Isan Journal of Pharmaceutical Sciences, 10, 25, 2015.
5. Inyai, C., Udomsin, O., Komaikul, J., Tanaka, H. Sritularak, B., Putalun, W.
“Enhamcement mulberroside A production in Morus alba L. cell cultures by calcium alginate immobilization and elicitation”
The 1st International Conference on Herbal and Traditional Medicine (HTM 2015), Khon Kaen, Thailand, January 28-30, 2015, O_04, Isan Journal of Pharmaceutical Sciences, 10, 27-32, 2015.
6. Limsuwanchote, S., Wungsintaweekul, J., Putalun, W., Udomsil, O., Yusakul, G., Tanaka, H.
“Preparation of polyclonal and monoclonal antibodies against mitragynine and the immunoassay development”
The 1st International Conference on Herbal and Traditional Medicine (HTM 2015), Khon Kaen, Thailand, January 28-30, 2015, O_19, Isan Journal of Pharmaceutical Sciences, 10, 261, 2015.
7. Komaikul, J., Kitisripanya, T., Udomsin, O., Tanaka, H. Sritularak, B., Putalun, W.
“Production and elicitation of mulberroside A from Morus alba cell suspension bioreactor”
The 1st International Conference on Herbal and Traditional Medicine (HTM 2015), Khon Kaen, Thailand, January 28-30, 2015, P_46, Isan Journal of Pharmaceutical Sciences, 10, 292, 2015.
1. 宇都 拓洋、坂元 政一、迫田 淳一、榮田 まり子、森永 紀、田中 宏幸、正山 征洋
“抗グリチルリチンモノクローナル抗体を機軸とした応用研究〜機能解析からミサイルタイプの分子育種〜”
第7回甘草に関す・驛Vンポジウム、九州大学(福岡)、2015年7月4日、P-03
2. 河野 俊享、清水 邦義、坂元 政一、田中 宏幸、森元 聡
“ELISA による霊芝トリテルペノイドganoderic acid A の迅速かつ高感度定量法の確立”
日本薬学会 第135年会、神戸学院大学他(兵庫)、2015年3月25−28日、28F-pm04
3. 常浦 祐未、松浦 ゆりの、米永 弥生、坂元 政一、麻生 真理子、黒瀬 等、田中 宏幸、森元 聡
“抗D-グルタミン酸モノクローナル抗体の作製とその特徴付け”
日本薬学会 第135年会、神戸学院大学他(兵庫)、2015年3月25−28日、28PB-am040S
2014年
総説等
1. Sakamoto, S., Shoyama, Y., Tanaka, H., Morimoto, S. “Application of green fluorescent protein in immunoassays” Adv. Biosci. Biotech., 5(6), 557-563, 2014.
原著論文
1. Sakamoto, S.*, Matsuura, Y.*, Yonenaga, Y., Tsuneura, Y., Aso, M., Kurose, H., Tanaka, H., Morimoto, S. *These authors contributed equally to this work. “Production and characterization of highly-specific monoclonal antibodies to D-glutamic acid” Monoclon. Antib. Immunodiagn. Immunother., 33(6), 414-419, 2014.
2. Limsuwanchote, S., Wungsintaweekul, J., Keawpradub, N., Putalun, W., Morimoto, S., Tanaka, H. “Development of indirect competitive ELISA for quantification of mitragynine in Kratom (Mitragyna speciosa (Roxb.) Korth.)” Forensic Sci. Int., 244(4), 70-77, 2014.
3. Komaikul, J., Kitisripanya, T., Tanaka, H., Sritularak, B., Putalun, W. “Development of an enzyme-linked immunosorbent assay for specific detection of mulberroside A in mulberry (Morus alba L.) using anti-mulberroside A polyclonal antibody” Food Anal. Methods, 7(1), 58-63, 2014.
4. Limsuwanchote, S., Wungsintaweekul, J., Yusakul, G., Han, J.Y., Sasaki-Tabata, K., Tanaka, H., Shoyama, Y., Morimoto, S. “Preparation of a monoclonal antibody against notoginsenoside R1, a distinctive saponin from Panax notoginseng, and its application to indirect competitive ELISA” Planta Med., 80(4), 337-342, 2014.
5. Ishii, Y., Koba, H., Kinoshita, K., Oizaki, T., Iwamoto, Y., Takeda, S., Miyauchi, Y., Nishimura, Y., Egoshi, N., Taura, F., Morimoto, S., Ikushiro, S., Nagata, K., Yamazoe, Y., Mackenzie, P.I., Yamada, H. “Alteration of the function of the UDP-glucuronosyltransferase 1A subfamily by cytochrome P450 3A4: different susceptibility for UGT isoforms and UGT1A1/7 variants” Drug Metab Dispos., 42(2), 229-238, 2014.
学会発表
(国際)1. Paudel, M.K., Sakamoto, S., Tanaka, H., Morimoto, S.
“Development of an immunoassay using anti-wogonin glucuronide monoclonal antibody”
The 8th JSP-CCTCNM-KSP Joint Symposium on Pharmacognosy, Fukuoka, Japan, September 13, 2014, SP-02
2. Kohno, T., Bang, T.H., Shimizu, K., Sakamoto, S., Tanaka, H., Morimoto, S.
“Tubulin-polymerizing activity of Ganoderma triterpenoids and production of their monoclonal antibody”
The 8th JSP-CCTCNM-KSP Joint Symposium on Pharmacognosy, Fukuoka, Japan, September 13, 2014, SP-76
3. Sakamoto, S., Putalun, W., Pongkitwitoon, B., Shoyama, Y., Tanaka, H., Morimoto, S.
“Enhanced plumbagin production in hairy root culture of Plumbago zeylanica by anti-plumbagin single-chain fragment antibody”
The 8th JSP-CCTCNM-KSP Joint Symposium on Pharmacognosy, Fukuoka, Japan, September 13, 2014, SP-17
1. 堀 太樹、坂元 政一、田中 宏幸、森元 聡
“ケシ由来パーオキシダーゼの機能解析”
日本生薬学会 第61回年会、福岡大学(福岡)、2014年9月13−14日、1C-1
2. 加藤 梨那、中村 俊介、相星 彩佳、坂元 政一、田畑 香織、田中 宏幸、森元 聡
“植物ネクローシスにおけるcyclophilin Dの機能解析と抗体作製”
日本生薬学会 第61回年会、福岡大学(福岡)、2014年9月13−14日、1C-3
3. 中島 由貴、田中 宏幸、坂元 政一、森元 聡
“植物アポトーシスにおけるシロイヌナズナ由来Cytochromecの機能解析”
日本生薬学会 第61回年会、福岡大学(福岡)、2014年9月13−14日、2P-90
4. 常浦 祐未、田畑 香織、坂元 政一、田中 宏幸、麻生 真理子、末宗 洋、森元 聡
“新規D-アミノ酸分析法開発を目指した抗D-セリンモノクローナル抗体の作製”
日本薬学会 第134年会、熊本大学(熊本)、2014年3月27−30日、28amM-040
5. 加藤 梨那、中村 俊介、相星 彩佳、坂元 政一、田畑 香織、田中 宏幸、森元 聡
“植物ネクローシスにおけるcyclophilin Dの機能解析”
日本薬学会 第134年会、熊本大学(熊本)、2014年3月27−30日、29amM-210
6. Komaikul, J., Kitisripanya, T., 田中 宏幸、Sritularak, B., Putalun, W.
“抗mulberroside Aポリクローナル抗体を用いたELISAによるクワ中mulberroside A分析法の開発”
日本薬学会 第134年会、熊本大学(熊本)、2014年3月27−30日、29amM-014
2008
総説等
1. 森元 聡
大麻の幻覚活性を制御する酵素
酵素工学ニュース(2008)
原著論文
1. C. Taguchi, F. Taura, T. Tamada, Y. Shoyama, Y. Shoyama, H. Tanaka, R. Kuroki and S.Morimoto, "Crystallization and preliminary X-ray diffraction studies of polyketide synthase-1 (PKS-1) from Cannabis sativa" Acta Cryst. F64, 217-220 (2008)
2. S. Sakamoto, W. Putalun, R. Tsuchihashi, S. Morimoto, J. Kinjo and H. Tanaka "Development of an enzyme-linked immunosorbent assay (ELISA) using higly-specific monoclonal antibodies against plambagin" Analytica Chimica Acta, 607, 100-105 (2008)
2007
総説等
1. F.Taura, S. Sirikantaramas, Y. Shoyama, Y.Shoyama and S. Morimoto "Phytocannabinoid in Cannabis sativa" Chemistry and Biodiversity, 4,1649-1663 (2007)
2. S. Sirikantaramas, F. Taura, S. Morimoto and Y. Shoyama "Recent advances in Cannabis satva research: Biosynthetic study and its potential in biotechnology" Current Pharmaceutical Biotechnology, 8, 237-343 (2007)
原著論文
1. S. Morimoto, Y. Tanaka, K. Sasaki, H. Tanaka, T.Fukamizu, Y. Shoyama, Y. Shoyama and F. Taura "Identification and characterization of cannabinoids that induce cell death through mitochondrial permeability transition in Cannabis leaf cells" J. Biol. Chem., 282, 20739-20751 (2007)
2. H. Ohtani, T. Ikegawa, Y. Honda, N. Koyama, S. Morimoto, Y. Shoyama, M. Juichi, M. Naito, T. Tsuruno, Y. Sawada "Effects of various methoxyflavones on vincristine uptake and multi-drug resistance to incristine in P-gp-over- expressing K562/ADM cells" Parmaceutical Res, 24, 1936-1943 (2007)
3. F. Taura, S.Sirikantaramas, Y.Shoyama, K.Yoshikai, Y.Shoyama, and S.Morimoto "CBDA synthase, chemotype-determining enzyme in fiber-type Cannabis sativa. " FEBS Letters, 581, 2929-2934 (2007)
4. F. Taura, E. Dono, S. Sirikantaramas, K. Yoshimura, Y. Shoyama, and S. Morimoto "Production of THCA by the biosynthetic enzyme from transgenic Pichia pastoris" Biochem. Biophys. Res. Commun., 361, 675-680 (2007)